The Laboratory Hamster And Gerbil

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Table of Contents

• Contents (p.7)
• Contributors (p.15)
• Preface (p.21)
• 1 Factors and steps to consider in setting up a cryopreservation protocol (p.25)
• 2 Applications of cryopreservation techniques in aquatic animals (p.34)
• 3 Cryopreservation of different biological materials to create Genome Resource Banks (p.38)
• 4 Examples of sperm cryopreservation in specific species (p.46)
• 5 Conclusions and future perspectives (p.56)
• References (p.58)
• 1 Introduction (p.73)
• 2 Conventional DNA evaluation methods (p.74)
• 3 DNA lesion detection by qPCR: A novel approach (p.77)
• 4 Our contribution to DNA damage evaluation in cryopreserved germ cells (p.80)
• 5 Specific protocol for DNA damage evaluation in zebrafish PGCs after cryopreservation (p.81)
• 6 Summary and future perspectives (p.86)
• References (p.87)
• 1 Introduction: Concept and importance of epigenetics in terms of evolution (p.93)
• 2 Epigenetic mechanisms (p.95)
• 3 The role of epigenetics in farmed fish species (p.98)
• 4 Epigenetic mechanisms in model species: A case study in zebrafish (p.102)
• 5 Techniques applied for the evaluation of DNA methylation (p.105)
• 6 Techniques applied for the evaluation of histone modifications (p.110)
• 7 Application of the epigenetic methods to our research (p.117)
• References (p.119)
• 1 Noncoding RNAs (p.129)
• 2 Biogenesis and mechanisms of action of miRNAs (p.131)
• 3 Methodological approaches applied in miRNA research (p.134)
• 4 miRNAs playing key roles in fish development and physiology (p.144)
• 5 Recent progress and future perspectives (p.166)
• References (p.170)
• 1 Introduction (p.185)
• 2 Epigenetic regulators (p.186)
• 3 Nutrients and metabolites influencing the epigenetic regulators (p.194)
• 4 Transfer of cell memory utilizing the epigenetic regulators (p.197)
• 5 Where do we go with nutritional epigenetics? (p.208)
• References (p.209)
• 1 Introduction (p.217)
• 2 Finding causative variants through QTL mapping approaches (p.218)
• 3 Detailed description of state-of-the-art methodology: Step by step on how to perform GWAS (p.224)
• 4 Pipeline (p.231)
• 5 The next generation of association mapping methods: Multimarker models (p.234)
• 6 Implementation of QTLs and causative variants into breeding programs (p.236)
• 7 Funneling candidate variants toward causality (p.237)
• 8 Conclusions (p.238)
• References (p.239)
• 1 The importance of gaining knowledge about the neuroendocrine system (p.245)
• 2 Neuroendocrine system (p.246)
• 3 Double-labeling immunofluorescence (p.249)
• 4 Going deeper on the pituitary hormone regulation (p.258)
• 5 How to approach this kind of neuroendocrine study in fish? (p.263)
• 6 Summary (p.266)
• References (p.267)
• 1 A brief introduction to the immune system in teleost fish (p.275)
• 2 Historical perspective and relevance of immunological studies in gilthead seabream (p.277)
• 3 Fish mucosal immunology (p.278)
• 4 How to alter or strengthen the fish mucosal immune system (p.286)
• 5 Methodologies to collect and preserve fish mucus (p.291)
• 6 Main questions still to be addressed (p.299)
• References (p.300)
• 1 Animal welfare (p.309)
• 2 The neuroendocrine stress response and its relation to welfare (p.312)
• 3 A proposed method to determine the stress status of fishes as part of the assessment of their welfare (p.315)
• 4 Conclusions and future perspectives (p.325)
• References (p.326)
• 1 Introduction (p.333)
• 2 Resources to study fish genome evolution (p.335)
• 3 Overview of fish genome evolution (p.337)
• 4 Evolutionary consequences of rediploidization—No “one size fits all” (p.342)
• 5 Transposable elements as agents of fish genome evolution (p.344)
• 6 Fish phylogenomics (p.346)
• 7 Evolution of “functional” regions in fish genomes (p.348)
• 8 A primer on miRNA evolutionary characterization (p.354)
• 9 Concluding remarks (p.360)
• References (p.360)
• 1 Introduction (p.371)
• 2 Larval zebrafish XT models of cancer (p.372)
• 3 Using the zebrafish for translational stem cell research (p.382)
• 4 Adult zebrafish XT models of cancer (p.387)
• 5 Conclusion (p.394)
• References (p.396)
• 1 A growing interest for cell lines of fish origin (p.405)
• 2 Current status of fish cell culture (p.407)
• 3 Applications for fish cell lines (p.409)
• 4 Fish cell lines capable of in vitro mineralization (p.413)
• 5 Development of bone-derived cells (p.418)
• 6 Perspectives for fish cell culture (p.421)
• References (p.423)
• Index (p.429)
• ... and 28 more chapters

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