Salmonella Methods And Protocols 2Nd Edition

Master Salmonella Methods And Protocols 2Nd Edition with this comprehensive guide. Essential reading for veterinary students, practitioners, and technicians seeking to deepen their expertise in Microbiology > Bacteriology > Salmonella, Methods and Protocols, 2nd Edition. Includes clinical case studies and practical applications. Instant digital download.

Table of Contents

• Preface (p.6)
• Contents (p.8)
• Contributors (p.10)
• 1 Luminex® Multiplex Bead Suspension Arrays for the Detection and Serotyping of Salmonella spp. (p.14)
• 1 Introduction (p.14)
• 2 Materials (p.19)
• 3 Methods (p.21)
• 4 Notes (p.36)
• References (p.40)
• 2 Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle (p.42)
• 1 Introduction (p.42)
• 2 Materials (p.44)
• 3 Methods (p.45)
• 4 Notes (p.52)
• References (p.58)
• 3 Determination of Antimicrobial Resistance in Salmonella spp. (p.60)
• 1 Introduction (p.60)
• 2 Materials (p.62)
• 3 Methods: Molecular-Biological Studies (p.63)
• 4 Notes (p.72)
• References (p.73)
• 4 Red-Mediated Recombineering of Salmonella enterica Genomes (p.76)
• 1 Introduction (p.76)
• 2 Materials (p.80)
• 3 Methods (p.81)
• 4 Further Variations of Red Recombineering (p.89)
• 5 Critical Parameters and Observations (p.90)
• 6 Concluding Remarks (p.90)
• 7 Notes (p.91)
• References (p.92)
• 5 A Method to Introduce an Internal Tag Sequence into a Salmonella Chromosomal Gene (p.94)
• 1 Introduction (p.94)
• 2 Materials (p.96)
• 3 Methods (p.97)
• 4 Validation of the 12023 sifA-2HA::Kmr Strain (p.102)
• 5 Notes (p.104)
• References (p.104)
• 6 Generation and Use of Site-Directed Chromosomal cyaA′ Translational Fusions in Salmonella enterica (p.106)
• 1 Introduction (p.106)
• 2 Materials (p.107)
• 3 Methods (p.109)
• 4 Notes (p.114)
• References (p.117)
• 7 Detection of Antimicrobial (Poly)Peptides with Acid Urea Polyacrylamide Gel Electrophoresis Followed by Western Immunoblot (p.118)
• 1 Introduction (p.118)
• 2 Materials (p.120)
• 3 Methods (p.122)
• 4 Notes (p.126)
• References (p.128)
• 8 Detecting Non-typhoid Salmonella in Humans by Enzyme-Linked Immunosorbent Assays (ELISAs): Practical and Epidemiological Aspects (p.130)
• 1 Serology and Salmonella Infections (p.130)
• 2 Suggested Protocol for a Standardized ELISA (Europe) (p.135)
• 3 Methods (p.135)
• 4 Notes (p.136)
• References (p.139)
• 9 Study of the Stn Protein in Salmonella; A Regulator of Membrane Composition and Integrity (p.140)
• 1 Introduction (p.140)
• 2 Materials (p.141)
• 3 Methods (p.143)
• 4 Notes (p.149)
• References (p.151)
• 10 Development of a Bacterial Nanoparticle Vaccine (p.152)
• 1 Introduction (p.152)
• 2 Materials (p.153)
• 3 Methods (p.155)
• 4 Notes (p.161)
• References (p.162)
• 11 Direct Attachment of Nanoparticle Cargo to Salmonella typhimurium Membranes Designed for Combination Bacteriotherapy Against Tumors (p.164)
• 1 Introduction (p.164)
• 2 Materials (p.169)
• 3 Method (p.171)
• 4 Notes (p.174)
• References (p.175)
• 12 Applications of Microscopy in Salmonella Research (p.178)
• 1 Introduction (p.178)
• 2 Materials (p.191)
• 3 Methods (p.193)
• 4 Notes (p.200)
• References (p.207)
• 13 Live Cell Imaging of Intracellular Salmonella enterica (p.212)
• 1 Introduction (p.212)
• 2 Materials (p.223)
• 3 Methods (p.225)
• 4 Notes (p.227)
• References (p.237)
• 14 In Vitro Modeling of Gallbladder-Associated Salmonella spp. Colonization (p.240)
• 1 Introduction (p.240)
• 2 Materials (p.241)
• 3 Methods (p.242)
• 4 Notes (p.247)
• References (p.248)
• 15 Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects (p.250)
• 1 Introduction (p.250)
• 2 Diversity of Lytic Phages Infecting the Genus Salmonella (p.252)
• 3 Diversity of Temperate Phages Infecting the Genus Salmonella (p.263)
• 4 Diversity of Salmonella Prophages (p.275)
• 5 Salmonella Phages: Practical Aspects (p.277)
• 6 Phagotherapy (p.281)
• 7 Concluding Remarks (p.288)
• References (p.290)
• Index (p.302)
• ... and 12 more chapters

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