Field And Laboratory Methods For Grassland And Animal Production Research

Field And Laboratory Methods For Grassland And Animal Production Research | File size: 7.5 MB | Format: PDF | Categories: General Books > Animal Production > Field and Laboratory Methods for Grassland and Animal Production Research

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Table of Contents

• Preface (p.6)
• Contents (p.8)
• Contributors (p.12)
• 1 Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre–loxP Site-Specific Recombination (p.18)
• 1 Introduction (p.18)
• 2 Materials (p.20)
• 3 Methods (p.24)
• 4 Notes (p.30)
• References (p.33)
• 2 Generation of Conditional Knockout Mice (p.36)
• 1 Introduction (p.36)
• 2 Materials (p.38)
• 3 Methods (p.40)
• 4 Notes (p.49)
• References (p.50)
• 3 Generating Mouse Lines for Lineage Tracing and Knockout Studies (p.52)
• 1 Introduction (p.52)
• 2 Materials (p.53)
• 3 Methods (p.56)
• 4 Notes (p.74)
• References (p.77)
• 4 Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE) (p.78)
• 1 Introduction (p.78)
• 2 Materials (p.86)
• 3 Methods (p.87)
• 4 Notes (p.89)
• References (p.90)
• 5 Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection (p.92)
• 1 Introduction (p.93)
• 2 Materials (p.94)
• 3 Methods (p.107)
• 4 Notes (p.120)
• References (p.124)
• 6 Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases (p.128)
• 1 Introduction (p.128)
• 2 Materials (p.136)
• 3 Methods (p.140)
• 4 Notes (p.148)
• References (p.153)
• 7 Intracytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice (p.156)
• 1 Introduction (p.156)
• 2 Materials (p.158)
• 3 Methods (p.161)
• 4 Notes (p.167)
• References (p.170)
• 8 Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice (p.172)
• 1 Introduction (p.172)
• 2 Materials (p.173)
• 3 Methods (p.176)
• 4 Notes (p.182)
• References (p.183)
• 9 Generation of Genetically Engineered Mice by the piggyBac Transposon System (p.186)
• 1 Introduction (p.186)
• 2 Materials (p.189)
• 3 Methods (p.191)
• 4 Notes (p.197)
• References (p.199)
• 10 Generation and Applications of MADM-Based Mouse Genetic Mosaic System (p.202)
• 1 Introduction (p.202)
• 2 Materials (p.205)
• 3 Methods (p.205)
• 4 Notes (p.211)
• References (p.216)
• 11 Generation of Mouse Lines Conditionally Over-expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System (p.218)
• 1 Introduction (p.218)
• 2 Materials (p.219)
• 3 Methods (p.226)
• 4 Notes (p.237)
• References (p.239)
• 12 In Situ Hybridization (Both Radioactive and Nonradioactive) and Spatiotemporal Gene Expression Analysis (p.240)
• 1 Introduction (p.240)
• 2 Materials (p.241)
• 3 Methods (p.243)
• 4 Notes (p.256)
• References (p.259)
• 13 Isolation and Handling of Mouse Embryonic Fibroblasts (p.262)
• 1 Introduction (p.262)
• 2 Materials (p.263)
• 3 Methods (p.263)
• 4 Notes (p.266)
• References (p.267)
• 14 Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue (p.268)
• 1 Introduction (p.268)
• 2 Materials (p.270)
• 3 Methods (p.274)
• 4 Conclusion (p.283)
• 5 Notes (p.284)
• References (p.285)
• 15 Pdx1 (GFP/w) Mice for Isolation, Characterization, and Differentiation of Pancreatic Progenitor Cells (p.286)
• 1 Introduction (p.286)
• 2 Materials (p.288)
• 3 Methods (p.291)
• 4 Notes (p.301)
• References (p.302)
• 16 Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development (p.304)
• 1 Introduction (p.304)
• 2 Materials (p.314)
• 3 Methods (p.316)
• 4 Notes (p.322)
• References (p.324)
• 17 Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System (p.328)
• 1 Introduction (p.328)
• 2 Materials (p.331)
• 3 Methods (p.333)
• 4 Notes (p.339)
• References (p.340)
• 18 Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo (p.342)
• 1 Introduction (p.342)
• 2 Materials (p.344)
• 3 Methods (p.346)
• 4 Notes (p.350)
• References (p.351)
• 19 Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts (p.356)
• 1 Introduction (p.356)
• 2 Materials (p.357)
• 3 Methods (p.359)
• 4 Notes (p.365)
• References (p.367)
• 20 Transgenic Nude Mice Ubiquitously Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment (p.368)
• 1 Introduction (p.368)
• 2 Materials (See Notes 1–3) (p.369)
• 3 Methods (p.371)
• 4 Notes (p.377)
• References (p.380)
• 21 Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty (p.382)
• 1 Introduction (p.382)
• 2 Materials (p.389)
• 3 Methods (p.391)
• 4 Notes (p.394)
• References (p.397)
• 22 Generating Double Knockout Mice to Model Genetic Intervention for Diabetic Cardiomyopathy in Humans (p.400)
• 1 Introduction (p.400)
• 2 Materials (p.404)
• 3 Methods (p.405)
• 4 Notes (p.410)
• References (p.414)
• 23 Experimental Osteoarthritis Models in Mice (p.416)
• 1 Introduction (p.417)
• 2 Materials (p.424)
• 3 Methods (p.426)
• 4 Notes (p.432)
• References (p.432)
• 24 Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI) (p.436)
• 1 Introduction (p.436)
• 2 Materials (p.439)
• 3 Methods (p.442)
• 4 Notes (p.448)
• References (p.449)
• 25 Mouse Models of Acute and Chronic Colitis (p.452)
• 1 Introduction (p.452)
• 2 Materials (p.453)
• 3 Methods (p.455)
• 4 Notes (p.462)
• References (p.463)
• 26 Manipulation and Assessment of Gut Microbiome for Metabolic Studies (p.464)
• 1 Introduction (p.464)
• 2 Materials (p.467)
• 3 Methods (p.470)
• 4 Notes (p.481)
• References (p.483)
• Index (p.486)
• ... and 38 more chapters

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