Transgenic Mouse Methods And Protocols 2Nd Edition

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Table of Contents

• Contents (p.9)
• 1 Introduction (p.13)
• 2 Science: Cells and Tissue Electroporation (p.16)
• 3 Technology (p.20)
• 4 New Development/Perspectives (p.22)
• 5 Conclusions (p.24)
• References (p.26)
• 1 Introduction (p.30)
• 2 Gross Biological Effects Observed in Electroporation (p.32)
• 3 Tissue Properties and Computation of Electric Field (p.37)
• 4 Electrode Designs and Configurations (p.44)
• 5 Pulse Parameters (Treatment Protocols) (p.53)
• 6 Numerical Prediction of Treatment Outcome (Treatment Planning) (p.55)
• 7 Conclusion (p.61)
• References (p.61)
• 1 History of Electrochemotherapy (p.70)
• 2 Electrochemotherapy in Veterinary Oncology (p.71)
• References (p.112)
• 1 Canine Cutaneous Mast Cell Tumors (p.120)
• 2 Feline Cutaneous Mast Cell Tumor (p.133)
• 1 Classical Treatment of Tumors of the Bladder and Urethra in Dogs (p.143)
• 2 Electrochemotherapy for the Treatment of Tumors in the Bladder and Urethra (p.144)
• 3 ECT Protocol for the Treatment of Bladder Tumors (p.144)
• 4 ECT Procedure for the Treatment of Bladder Tumors (p.145)
• 5 Follow-Up of Patients Who Underwent ECT for the Treatment of Bladder Tumors (p.145)
• 6 Treatment with COX Inhibitors in Patients Undergoing ECT for the Treatment of Bladder Tumors (p.146)
• 7 ECT Outcomes for Bladder Tumors (p.147)
• References (p.147)
• 1 Introduction (p.151)
• 2 Calcium Electroporation and How It Is Carried Out (p.151)
• 3 Calcium Electroporation: Background (p.155)
• 4 Human Clinical Trials (p.162)
• 5 Veterinary Studies with Calcium Electroporation (p.163)
• 6 Perspectives (p.166)
• 7 Conclusion (p.167)
• References (p.167)
• 1 Irreversible Electroporation Theory and Techniques (p.171)
• 2 Treatment Planning (p.175)
• 3 Limitations of IRE and H-FIRE (p.177)
• 4 Clinical Applications of IRE and H-FIRE in Companion Animals with Naturally Occurring Cancers (p.179)
• 5 Clinical Applications of IRE and H-FIRE in Preclinical Normal Animal Models or Experimentally Induced Tumors (p.191)
• 6 Summary (p.198)
• References (p.198)
• 1 Combination with Surgery (p.211)
• 2 Combination with Chemotherapy (p.216)
• 3 Combination with Radiotherapy (p.217)
• 4 Combination with Cryosurgery (p.218)
• References (p.219)
• 1 Introduction (p.223)
• 2 Mechanism of Gene Electrotransfer (p.224)
• 3 Preclinical Use of GET (p.225)
• 4 Clinical Development for Oncology Applications (p.230)
• 5 GET Veterinary Clinical Trials (p.233)
• 6 Summary (p.234)
• References (p.234)
• 1 Cancer Vaccines and GET (p.240)
• 2 Genetic Cancer Vaccines and Tumor-Associated Antigens (p.241)
• 3 Preclinical Studies (p.243)
• 4 DNA-GET in Human Clinical Trials (p.245)
• 5 DNA-GET in Veterinary Clinical Trials (p.249)
• 6 Conclusions (p.252)
• References (p.253)
• 1 Introduction (p.258)
• 2 IL-12 Gene Electrotransfer (p.260)
• 3 Future Directions and Conclusions (p.271)
• References (p.276)
• 1 Canine Malignant Melanoma and Osteosarcoma: An Overview (p.283)
• 2 Immunotherapeutic Approaches in Canine Malignant Melanoma and Osteosarcoma Oncology (p.286)
• 3 DNA Vaccines (p.293)
• 4 Application of Electroporation to DNA Vaccination (p.295)
• 5 Case Study: DNA Vaccination with In Vivo Electroporation in Canine Oral Malignant Melanoma and Osteosarcoma (p.297)
• 6 Conclusions (p.301)
• References (p.302)
• 1 Introduction (p.311)
• 2 The Electrodes (p.312)
• 3 Single Needle Electrode (p.312)
• 4 The Curved Electrode (p.314)
• 5 SN-HFIRE Electrode (p.315)
• 6 Brain Electrode (p.315)
• 7 Endo-Ve (p.317)
• 8 Pulsed-Field Ablation System for Cardiac Ablation (p.318)
• References (p.320)
• 1 Background (p.324)
• 2 ECT in Preclinical Studies of Deep Tumors (p.325)
• 3 The Expandable and Deployable Electrodes (p.326)
• 4 Bipolar Electrodes (p.330)
• 5 Treatment Planning (p.334)
• 6 Conclusions (p.336)
• References (p.337)
• 1 Background (p.342)
• 2 Concepts of Electroporation Systems (p.344)
• 3 Generator Structure (p.345)
• 4 Non-Tumor Electroporation Applications (p.350)
• 5 Conclusions (p.353)
• References (p.353)
• ... and 47 more chapters

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