Protocols For The Diagnosis Of Pig Viral Diseases

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Table of Contents

• Preface (p.6)
• Contents (p.7)
• Contributors (p.12)
• 1 Introduction (p.16)
• 2 Infectious Disease Outbreak and Its Spread (p.22)
• 3 Epidemiological ``Know-How´´: An Important Pillar for Development of Surveillance System and Outbreak Preparedness (p.22)
• 4 Investigation of Viral Disease Outbreak in Swine (p.23)
• 5 Outbreak Investigation Steps for Infectious Diseases (p.27)
• 6 Implementation of Viral Disease Control Program at Various Levels (p.30)
• 7 Prevention and Control Measures for the Viral Disease Outbreaks in Swine Population (p.31)
• 8 Conclusion (p.32)
• References (p.33)
• 1 Background (p.36)
• 2 Introduction (p.37)
• 3 Suitable Criteria for Collection and Transport and Preservation of Samples (p.38)
• 4 Collection and Transport and Preservation of Samples (p.42)
• 5 Conclusion (p.44)
• References (p.44)
• 1 Introduction (p.46)
• 2 Infectivity Assays (p.47)
• 3 Calculation of End-Point Titer (p.51)
• 4 Haemadsorption Assay-Based Infectivity Assay for African Swine Fever Virus (ASFV) (p.54)
• 5 Chemical/Physical Methods of Virus Quantitation (p.54)
• 6 Applications of Virus Quantification (p.58)
• 7 Conclusion (p.59)
• References (p.60)
• 1 Introduction (p.63)
• 2 Important Considerations for Purification of the RNA from Clinical Specimen (p.65)
• 3 Lysis Buffer (p.66)
• 4 Density Gradient Centrifugation (p.69)
• 5 Silica Technology (p.72)
• 6 Affinity Matrices (p.75)
• 7 Purification Using Magnetic Beads (p.75)
• 8 Separation of Double-Stranded RNA from Single-Stranded RNA (p.76)
• 9 Filter Paper Matrix Cards (p.78)
• 10 Storage of Purified RNA (p.78)
• References (p.79)
• 1 Introduction (p.81)
• 2 Principle of Multiplex PCR (p.82)
• 3 Optimization of a Multiplex PCR (p.86)
• 4 Multiplex PCR Troubleshooting (p.93)
• 5 Key Pointers for Multiplex PCR Development (p.95)
• 6 Evaluation as a Potential Diagnostic Tool (p.95)
• 7 Advantages of Multiplex PCR (p.99)
• 8 Application of Multiplex PCR in Porcine Diseases (p.99)
• 9 Conclusion (p.102)
• References (p.102)
• 1 Introduction (p.105)
• 2 Materials (p.107)
• 3 Methods of Plasmid Isolation (p.109)
• 4 Notes (p.119)
• References (p.120)
• 1 Introduction (p.122)
• 2 Materials (p.129)
• 3 Methods (p.129)
• 1 Introduction (p.140)
• 2 RNA-PAGE Principle (p.141)
• 3 Sample Preparation: RNA Extraction from Clinical or Biological Samples (p.142)
• 4 Procedure: From Setting Up PAGE Apparatus to Stained Gels Visualization (p.143)
• 5 Method (p.143)
• References (p.149)
• 1 Introduction (p.150)
• 2 Peptide Nucleic Acid (PNA) (p.151)
• 3 Materials and Methods (p.151)
• References (p.161)
• 1 Introduction (p.163)
• 2 Loop-Mediated Isothermal Amplification (LAMP) (p.165)
• 3 Nucleic Acid Sequence-Based Amplification (NASBA) (p.165)
• 4 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) (p.166)
• References (p.168)
• 1 Introduction (p.170)
• 2 Selection of Aptamers (p.171)
• 3 Application of Aptamer in Detection of Viral Diseases (p.172)
• 4 Nucleic Acid Aptamers (p.173)
• 5 Peptide Aptamers (p.175)
• 6 Peptide Nucleic Acid Aptamers (p.175)
• 7 Advantages of Aptamers (p.176)
• 8 Future Prospects (p.177)
• References (p.178)
• 1 Introduction (p.181)
• 2 General Structure of an Antibody: An Overview (p.183)
• 3 Biosensor/Immunosensor (p.186)
• 4 Antibody Immobilization (p.188)
• 5 Biosensor Types (p.191)
• 6 Biosensor Fabrication (p.197)
• 7 Methods for Biosensor Fabrication and Analyte Detection (p.199)
• References (p.201)
• 1 Introduction (p.204)
• 2 LFA Design and Principle (p.206)
• 3 Materials (p.208)
• 4 Methods (p.208)
• 5 Notes (p.211)
• References (p.212)
• 1 Introduction (p.214)
• 2 ddPCR Experimental Workflow (p.216)
• 3 Notes (p.218)
• 4 ddPCR for Porcine Viruses (p.220)
• 5 Conclusion (p.221)
• References (p.221)
• 1 Introduction (p.223)
• 2 Materials (p.226)
• 3 Method (p.226)
• 4 Notes (p.232)
• 5 Conclusion (p.236)
• References (p.236)
• 1 Introduction (p.238)
• 2 Materials Required for PSR (p.240)
• 3 Methods (p.241)
• 4 Notes (p.244)
• References (p.244)
• 1 Introduction (p.246)
• 2 RPA Mechanism (p.248)
• 3 RPA Operating Parameters (p.248)
• 4 Detection of Amplicons (p.252)
• 5 RPAs for Porcine Viruses (p.254)
• 6 Conclusion (p.255)
• References (p.255)
• 1 Introduction (p.258)
• 2 Types of Cell Culture System (p.259)
• 3 Cell Culture System for Porcine DNA Viruses (p.260)
• 4 Cell Culture System for Porcine RNA Viruses (p.262)
• References (p.267)
• 1 Monoclonal Antibody (mAb) (p.271)
• 2 How Hybridomas Are Produced? (p.272)
• 3 Characterization of mAb (p.277)
• 4 Application of mAbs in Diagnosis (p.277)
• 5 Conclusion (p.278)
• References (p.279)
• 1 Introduction (p.281)
• 2 Sample Collection (p.282)
• 3 Sample Preparation (p.283)
• 4 Probes (p.284)
• 5 Optimizing Conditions for Hybridization (p.286)
• 6 Nucleic Acid Hybridization Protocol (p.288)
• 7 Advantages and Limitations of Nucleic Acid Hybridization Methods (p.291)
• 8 Applications of Nucleic Acid Hybridization in Viral Disease Diagnosis (p.292)
• 9 Conclusion (p.293)
• References (p.293)
• 1 Introduction (p.296)
• 2 Principles of LDR (p.297)
• 3 Principles of LDR-Fluorescent Microsphere Assay (LDR-FMA) (p.298)
• 4 Materials (p.299)
• 5 Methods (p.299)
• References (p.300)
• 1 Introduction (p.302)
• 2 Materials (p.308)
• 3 Methods (p.311)
• 4 Notes (p.314)
• References (p.315)
• 1 Introduction (p.318)
• 2 Sample Preparation (p.319)
• 3 Electrophoresis (p.320)
• 4 SDS-PAGE (p.320)
• 5 Native PAGE (p.321)
• 6 Protocol for SDS-PAGE (p.322)
• 7 Protocol for Western Blot (p.327)
• 8 Applications of PAGE and Western Blotting in Virology (p.328)
• 9 Limitations of Western Blot (p.329)
• 10 Troubleshooting (p.330)
• 11 Conclusion (p.332)
• References (p.332)
• 1 Introduction (p.334)
• 2 Viral Diseases of Pigs and Their Public Health Importance (p.336)
• 3 Diagnostic Methods for Viral Diseases: Needs and Challenges (p.337)
• 4 Recent Methods in the Diagnosis of Viral Infections (p.341)
• 5 Immune Assays in Swine Viral Diseases (p.344)
• 6 Immunoassays and Immunochemistry (p.345)
• 7 Swine Viral Diseases and Major Diagnostics Immunoassays (p.346)
• 8 Conclusion (p.352)
• References (p.353)
• 1 Introduction (p.355)
• 2 Materials (p.357)
• 3 Methods (p.357)
• 4 Notes (p.363)
• References (p.364)
• 1 Introduction (p.366)
• 2 History of Lab-Escaped Pathogens (p.367)
• 3 Good Laboratory Practices (p.369)
• 4 Biosafety Requirements While Working with Infectious Agents (p.370)
• 5 Risk Groups, Biosafety Levels, and Physical Containment Levels (p.373)
• 6 Working Safely in a Virology Laboratory (p.374)
• 7 Conclusion (p.378)
• References (p.378)
• Index (p.381)
• ... and 1 more chapters

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