High-Quality, High-Volume Spay and Neuter and Other Shelter Surgeries

High-Quality, High-Volume Spay and Neuter and Other Shelter Surgeries

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Comprehensive veterinary reference covering high-quality, high-volume spay and neuter and other shelter surgeries. This digital edition provides in-depth coverage suitable for veterinarians, veterinary students, technicians, and animal health professionals.

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• Instant digital download after purchase
• High-quality PDF format - works on all devices
• Fully searchable text for quick clinical reference
• Printable pages for study and practice use

Category: Veterinary Medicine

Published by Global Vet and Co Ltd.

Table of Contents

• Preface (p.8)
• Contents (p.12)
• Contributors (p.16)
• 1 Overview and Challenges of Molecular Technologies in the Veterinary Microbiology Laboratory (p.24)
• 1 Introduction (p.24)
• 2 Molecular Diagnostics Market (p.26)
• 3 Brief Overview of Molecular Technologies in the Veterinary Microbiology Laboratory (p.26)
• 4 SWOT Analysis of Molecular Diagnostics Technologies (p.29)
• 5 Conclusion (p.35)
• References (p.37)
• 2 Significance and Integration of Molecular Diagnostics in the Framework of Veterinary Practice (p.40)
• 1 Introduction (p.40)
• 2 Basic Approaches in the Laboratory (p.41)
• 3 Level of Information (p.42)
• 4 The Technique That Is Best Suited for the Laboratory (p.44)
• 5 Limitations of the Molecular Techniques (p.46)
• 6 Technologies in the Near Future (p.48)
• 7 Conclusion (p.49)
• References (p.50)
• 3 Biosafety Principles and Practices for the Veterinary Diagnostic Laboratory (p.52)
• 1 Introduction (p.52)
• 2 Basic Elements of a Biosafety Program (p.53)
• 3 Risk Assessment for Veterinary Diagnostic Labs (p.55)
• 4 Biocontainment Levels (p.58)
• 5 Minimal Facility Requirements for All Biological Laboratory Work (p.59)
• 6 Operational Procedures (Emphasis on Molecular Testing) (p.59)
• 7 Conclusion (p.62)
• References (p.62)
• 4 Veterinary Biobank Facility: Development and Management for Diagnostic and Research Purposes (p.64)
• 1 Introduction (p.64)
• 2 Development of a Veterinarian Biobank Infrastructure (p.66)
• 3 Sample Types and Preparation (p.70)
• 4 Storage of Biological Resources (p.75)
• 5 Application of Biological Resources (p.75)
• 6 Role of Biobanks in Veterinary Medicine (p.77)
• 7 Remarks and Future Perspectives (p.78)
• References (p.80)
• 5 Biological Specimen Collection and Processing for Molecular Analysis (p.82)
• 1 Introduction (p.82)
• 2 Materials (p.85)
• 3 Methods (p.90)
• 4 Notes (p.93)
• References (p.96)
• 6 Validation of Molecular Diagnostic Assays and Quality Assurance and Control in the Veterinary Laboratory (p.98)
• 1 Introduction (p.98)
• 2 Validation of Molecular Diagnostics (p.99)
• 3 Quality Assurance (p.101)
• 4 Quality Control (p.102)
• 5 Planning and Inception (p.105)
• 6 Assay Validation Plan (p.107)
• 7 Validation Design (p.108)
• 8 Performing the Validation (p.111)
• 9 Risk Assessment (p.119)
• 10 Data Analysis and Composition of the Technical Report (p.120)
• 11 Review of Technical Report and Sign-Off (p.120)
• 12 Instructions for Use and Labeling (p.120)
• 13 Monitoring the Performance of Assays and Maintenance of the Validation File (p.120)
• 14 Production of Reagents (p.121)
• 15 “Rollout” of Assays (p.122)
• 16 “Post-Deployment” Surveillance and Verification (p.122)
• 17 Conclusions (p.124)
• Glossary of Terms (p.125)
• References (p.126)
• 7 Molecular Approaches to Recognize Relevant and Emerging Infectious Diseases in Animals (p.130)
• 1 Introduction (p.130)
• 2 PCR-Based Approaches (p.132)
• 3 Isothermal Amplification (p.135)
• 4 Detection by Hybridization-Based Approaches (p.136)
• 5 Further Trends, New Tools in Molecular Diagnostics (p.138)
• 6 Summary and Final Remarks (p.140)
• References (p.142)
• 8 Real-Time Reverse Transcriptase PCR for the Detection of Bluetongue Virus (p.146)
• 1 Introduction (p.146)
• 2 Materials (p.148)
• 3 Methods (p.149)
• 4 Notes (p.150)
• References (p.152)
• 9 Nested and Multiplex Real-Time PCR Using Dual-Labeled Probes: Detecting and Discriminating Mycobacterium tuberculosis Complex Members in Cultures and Animal Tissues (p.154)
• 1 Introduction (p.154)
• 2 Materials (p.156)
• 3 Methods (p.156)
• 4 Notes (p.161)
• References (p.163)
• 10 A Real-Time PCR Assay for the Diagnosis of Gastrointestinal Nematode Infections of Small Ruminants (p.166)
• 1 Introduction (p.166)
• 2 Materials (p.168)
• 3 Methods (p.168)
• 4 Notes (p.170)
• References (p.173)
• 11 Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches (p.174)
• 1 Introduction (p.174)
• 2 Materials (p.176)
• 3 Methods (p.177)
• 4 Notes (p.180)
• References (p.182)
• 12 Detection of Fish Pathogens by Loop-Mediated Isothermal Amplification (LAMP) Technique (p.184)
• 1 Introduction (p.184)
• 2 Materials (p.186)
• 3 Methods (p.188)
• 4 Notes (p.191)
• References (p.194)
• 13 Direct Detection of Theileria annulata in Bovine Blood Samples Using Standard and Isothermal DNA Amplification Approaches (p.196)
• 1 Introduction (p.196)
• 2 Materials (p.197)
• 3 Methods (p.198)
• 4 Notes (p.200)
• References (p.203)
• 14 Reverse Line Blot Hybridization with Species-Specific Oligonucleotide Probes: Application to Piroplasm Detection (p.204)
• 1 Introduction (p.204)
• 2 Materials (p.206)
• 3 Methods (p.207)
• 4 Notes (p.211)
• References (p.212)
• 15 DNA Microarray-Based Detection of Multiple Pathogens: Mycoplasma spp. and Chlamydia spp. (p.214)
• 1 Introduction (p.214)
• 2 Materials (p.219)
• 3 Methods (p.221)
• 4 Notes (p.226)
• References (p.228)
• 16 In Situ Hybridization with Labeled Probes: Assessment of African Swine Fever Virus in Formalin-Fixed Paraffin-Embedded Tissues (p.230)
• 1 Introduction (p.230)
• 2 Materials (p.231)
• 3 Methods (p.233)
• 4 Notes (p.235)
• References (p.238)
• 17 Fluorescence In Situ Hybridization for the Tissue Detection of Bacterial Pathogens Associated with Porcine Infections (p.240)
• 1 Introduction (p.240)
• 2 Materials (p.246)
• 3 Methods (p.248)
• 4 Notes (p.253)
• References (p.254)
• 18 Identification of Animal Pasteurellaceae by MALDI-TOF Mass Spectrometry (p.256)
• 1 Introduction (p.256)
• 2 Materials (p.258)
• 3 Methods (p.259)
• 4 Notes (p.262)
• References (p.264)
• 19 Gold Nanoparticles as a Potential Tool for Diagnosis of Fish Diseases (p.266)
• 1 Introduction (p.266)
• 2 Materials (p.269)
• 3 Methods (p.269)
• 4 Notes (p.271)
• References (p.272)
• 20 Nucleic-Acid Testing, New Platforms and Nanotechnology for Point-of-Decision Diagnosis of Animal Pathogens (p.274)
• 1 The Context of Disease Diagnosis in Veterinary Science, Medicine, and Practice (p.275)
• 2 Nucleic-Acid Testing (p.283)
• 3 Methods for Signal Transduction in Biosensors and Point-of-Care Devices (p.287)
• 4 New Platforms for Point-of-Care Disease Diagnosis (p.289)
• 5 Nanotechnology (p.294)
• 6 Conclusions (p.299)
• References (p.300)
• 21 Molecular Typing Tools: From Pattern Recognition to Genome-Based Algorithms (p.308)
• 1 Introduction (p.308)
• 2 Fingerprint Typing Methods (p.309)
• 3 Typing Based on Repetitive Elements (p.314)
• 4 Single-Locus Sequence Typing (p.316)
• 5 Multi-locus Sequence Typing (MLST) (p.317)
• 6 Genome-Wide Typing Approaches (p.319)
• 7 Conclusion (p.322)
• References (p.322)
• 22 Characterization of Campylobacter jejuni and Campylobacter coli Genotypes in Poultry Flocks by Restriction Fragment Length Polymorphism (RFLP) Analysis (p.332)
• 1 Introduction (p.332)
• 2 Materials (p.334)
• 3 Methods (p.336)
• 4 Notes (p.339)
• References (p.342)
• 23 Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci (p.344)
• 1 Introduction (p.344)
• 2 Materials (p.345)
• 3 Methods (p.348)
• 4 Notes (p.354)
• References (p.355)
• 24 Multiple-Locus Variable-Number Tandem Repeat (VNTR) Analysis (MLVA) Using Multiplex PCR and Multicolor Capillary Electrophoresis: Application to the Genotyping of Brucella Species (p.356)
• 1 Introduction (p.356)
• 2 Materials (p.357)
• 3 Methods (p.361)
• 4 Notes (p.367)
• References (p.368)
• 25 Multilocus Sequence Typing (MLST): Markers for the Traceability of Pathogenic Leptospira Strains (p.370)
• 1 Introduction (p.370)
• 2 Materials (p.372)
• 3 Methods (p.375)
• 4 Notes (p.377)
• References (p.380)
• 26 Single-Nucleotide Polymorphism Discrimination Using High-Resolution Melting Analysis for the Genotyping of Bacillus anthracis (p.382)
• 1 Introduction (p.382)
• 2 Materials (p.384)
• 3 Methods (p.386)
• 4 Notes (p.389)
• References (p.392)
• 27 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) Analysis of Members of the Mycobacterium tuberculosis Complex (p.394)
• 1 Introduction (p.394)
• 2 Materials (p.400)
• 3 Methods (p.403)
• 4 Notes (p.407)
• References (p.409)
• 28 Rapid Microarray-Based Genotyping of Chlamydia spp. Strains from Clinical Tissue Samples (p.412)
• 1 Introduction (p.412)
• 2 Materials (p.415)
• 3 Methods (p.417)
• 4 Notes (p.419)
• References (p.420)
• 29 Multiplexed Genotyping of Bacillus anthracis by Luminex xMap Suspension Array (p.422)
• 1 Introduction (p.422)
• 2 Materials (p.425)
• 3 Methods (p.429)
• 4 Notes (p.431)
• References (p.433)
• 30 Next-Generation Sequencing in Veterinary Medicine: How Can the Massive Amount of Information Arising from High-Throughput Technologies Improve Diagnosis, Control, and Management of Infectious Diseases? (p.436)
• 1 Introduction (p.437)
• 2 Massive Parallel Sequencing (p.438)
• 3 Application of NGS to Animal Infectious Disease (p.442)
• 4 Conclusions (p.449)
• References (p.451)
• 31 Impact of Next-Generation Technologies on Exploring Socioeconomically Important Parasites and Developing New Interventions (p.458)
• 1 Introduction (p.458)
• 2 Brief Background on Parasitic Nematodes (p.459)
• 3 Some Key Techniques for Transcriptomic Investigations of Parasitic Nematodes (p.465)
• 4 Caenorhabditis elegans as Major Resource for Comparative Studies (p.472)
• 5 Understanding Nematodes of Socioeconomic Importance Through Genomics and Transcriptomics: Examples (p.473)
• 6 Opportunities for Drug Discovery Using Global Datasets (p.476)
• 7 Challenges and Prospects (p.479)
• 8 Update on Next-Generation Sequencing Technologies (p.482)
• References (p.483)
• 32 Functional Genomics of Tick Vectors Challenged with the Cattle Parasite Babesia bigemina (p.496)
• 1 Introduction (p.496)
• 2 Materials (p.499)
• 3 Methods (p.501)
• 4 Notes (p.507)
• References (p.509)
• 33 Metagenomic Approaches to Disclose Disease-Associated Pathogens: Detection of Viral Pathogens in Honeybees (p.512)
• 1 Introduction (p.512)
• 2 Materials (p.514)
• 3 Methods (p.518)
• 4 Notes (p.528)
• References (p.531)
• 34 Proteomics Characterization of Tick-Host-Pathogen Interactions (p.534)
• 1 Introduction (p.534)
• 2 Materials (p.535)
• 3 Methods (p.538)
• 4 Notes (p.545)
• References (p.547)
• Index (p.550)
• ... and 11 more chapters

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